FIGURE 3.

Proposed differentiation therapy for osteosarcoma cancer stem cells. Osteosarcoma is a heterogeneous tissue that includes AX‐type cells, AO‐type cells, chondrogenic differentiated cells, and osteogenic differentiated cells (A). We propose a differentiation‐based therapeutic strategy for osteosarcoma in which treatment with conventional anticancer drugs eliminates AX‐type tumor cells (B) and then treatment with a Rho‐kinase (ROCK) or megakaryoblastic leukemia 1 (MKL1) inhibitor induces terminal adipocyte differentiation of AO‐type chemoresistant stemlike cells through modulation of the actin cytoskeleton (C)