FIGURE 1.

IL‐1R is overexpressed in malignant pleural mesothelioma (MPM) cells compared with mesothelial cells. A, Comprehensive analysis of IL‐1β expression in human mesothelial cell (Met‐5A) and MPM cell lines (MSTO‐211H, H2452, H2052, and H28) by immunoblotting. Cell lysates from THP‐1 cells were loaded as a positive control. Expression of IL‐1β was detected neither in mesothelial cell nor in MPM cells. B, Comprehensive analysis of IL‐1β expression in human mesothelial cell and MPM cell lines by qRT‐PCR. Expression of IL‐1β transcripts was less in all the MPM cells than in mesothelial cells. C, Comprehensive analysis of IL‐1R expression in human mesothelial cells and MPM cells lines by qRT‐PCR. Expression of IL‐1R was higher in MPM cells than in mesothelial cells. D, E, Protein level of IL‐1R in human mesothelial cell (Met‐5A) and MPM cell (H2452) was examined by immunoblotting and immunofluorescence. Consistent with the results of qRT‐PCR and immunoblotting, expression of IL‐1R was higher in H2452 cells. All the above experiments were performed at least twice with similar results. Representative data are shown. Scale Bars, 100 μm