FIGURE 4.

Complex formation of extracellular matrix metalloproteinase inducer (emmprin)‐CD73 complex, and the role of CD73 in MMP‐2 production when CD73 siRNA was transfected into fibroblasts cocultured with squamous cell carcinoma cells. Arrow, CD73; arrowhead, emmprin; asterisk, emmprin‐CD73 complex. The lower‐molecular‐weight signals in the blots indicate immunoglobulins. A, Blots were probed for CD73 or emmprin expression following immunoprecipitation of emmprin or CD73 in ST353i cells cocultured with A431 cells. B, Blots were probed for CD73 or emmprin expression following immunoprecipitation of emmprin or CD73 in ST353i cells cocultured with CRL‐2095 cells. C, The immunoblot (IB) represents CD73 expression when CD73 siRNA was transfected in ST353i cells cocultured with A431 cells. D, The IB represents CD73 expression when CD73 siRNA was transfected in ST353i cells cocultured with CRL‐2095 cells. E, The IB represents MMP‐2 production when CD73 siRNA was transfected in ST353i cells cocultured with A431 cells. F, The IB represents MMP‐2 production when CD73 siRNA was transfected in ST353i cells co‐cultured with CRL‐2095 cells. BS3, bis(sulfosuccinimidyl)suberate