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Mass spectra—product ion scan confirming glycosylation in mutant 76E4 K275L with (A) UDP-Glc, (B) UDP-Gal and (C) UDP-GlcNAc donor sugars; (D) GAR screen results showing summary of mutant UGTs 76E4 K275L, 76D1 P129T, 76D1 G347C, and 76E2 D374E donor activities where green and red indicate positive and no activity, respectively.
