Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Thermus thermophilus) |
HB8, ATCC 27634, DSM 579 |
Uniprot | Nqo12: Q56227 Nqo13: Q56228 Nqo14: Q56229 |
Multiple sequence alignments |
| Gene (Pyrococcus furiosus) |
COM1, DSM 3638 |
pdb FASTA sequence |
6CFW | Multiple sequence alignments |
| Gene (Bacillus pseudofirmus) |
Strain OF4 | Uniprot | MrpA: Q9RGZ5 MrpB: Q9RGZ4 MrpC: Q9RGZ3 MrpD: Q9RGZ2 MrpE: Q9RGZ1 MrpF: Q9RGZ0 MrpG: Q9RGY9 |
Multiple sequence alignments |
| Gene (Bacillus subtilis) |
168 | Uniprot | MrpA: Q9K2S2 MrpB: O05259 MrpC: O05260 MrpD: O05229 MrpE: Q7WY60 MrpF: O05228 MrpG: O05227 |
Multiple sequence alignments |
| Gene (Staphylococcus aureus) |
1280 | Uniprot | MrpA: Q9ZNG6 MrpB: P60678 MrpC: P60682 MrpD: P60686 MrpE: P60690 MrpF: P60694 MrpG: P60698 |
Multiple sequence alignments |
| Gene (Thermosynechococcus elongatus) |
BP-1 | Uniprot | NdhF1: Q8DKX9 NdhD1: Q8DKY0 |
Multiiple sequence alignments |
| Sequence-based reagent | MrpF D35L | This paper | PCR primers |
Forward:
CGCTTATTTTTTACTATATATGTTGAAAAAAAATGAAAC
Reverse: GCAAGCGTAATGCCCATCGCTAAGAGCGCGATAATACGATCCG Forward: CGGATCGTATTATCGCGCTCTTAGCGATGGGCATTACGCTTGC Reverse: ATATAGTAAAAAATAAGCG |
| Sequence-based reagent | MrpG S72W | This paper | PCR primers |
Forward:
CGCTTATTTTTTACTATATATGTTGAAAAAAAATGAAAC
Reverse: CACGATGCCAAGCAATAGACGCCAGTTGAAATGGTTATTTTCAATG Forward: CATTGAAAATAACCATTTCAACTGGCGTCTATTGCTTGGCATCGTG Reverse: ATATAGTAAAAAATAAGCG |
| Sequence-based reagent | MrpE L41W | This paper | PCR primers |
Forward:
CGCTTATTTTTTACTATATATGTTGAAAAAAAATGAAAC
Reverse: GCGCGAATGGAAAAAGCGACGCCATATAAAAAGAATAAACAGCCCGATCATGTAC Forward: GTACATGATCGGGCTGTTTATTCTTTTTATATGGCGTCGCTTTTTCCATTCGCGC Reverse: ATATAGTAAAAAATAAGCG |
| Strain, strain background (Escherichia coli) | KNabc | doi:10.1073/pnas.84.9.2615 | Expression and assay strain | |
| Transfected construct (Anoxybacillus flavithermus) | DSM 21510/WK1 | Other | Uniprot MrpA: B7GL84 MrpB: B7GL83 MrpC: B7GL82 MrpD: B7GL98 MrpE: B7GL97 MrpF: B7GL96 MrpG: B7GIG3 |
Prof. Masahiro Ito (Graduate School of Life Sciences, Toyo University, Japan) |
| Software, algorithm | SerialEM | doi:10.1016/j.jsb.2005.07.007 | Data Acquisition Software for a variety of data from electron microscopes | |
| Software, algorithm | Relion | doi:10.1016/j.jsb.2012.09.006 | Cryo-EM processing software | |
| Software, algorithm | CTFFIND4 | doi:10.1016/j.jsb.2015.08.008 | Defocus estimation software | |
| Software, algorithm | Gctf | doi:10.1016/j.jsb.2015.11.003 | Per-particle CTF estimation software | |
| Software, algorithm | USCF Chimera | doi:10.1002/jcc.20084 | Visualisation software of molecular structures and cryo-EM maps | |
| Software, algorithm | Coot | doi:10.1107/S0907444904019158 | Software for model building | |
| Software, algorithm | PHENIX | doi:10.1107/S0907444909052925 | Structure refinement software | |
| Software, algorithm | MotionCor2 | doi:10.1038/nmeth.4193 | Whole frame image motion correction software |