Table 1. Cryo-EM data collection, refinement and validation statistics.
| Data collection and processing | ||
|---|---|---|
| Magnification | 105000 | |
| Voltage (kV) | 300 | |
| Electron exposure (e-/Å2) | 85 | |
| Defocus range (μm) | 0.6–2.3 | |
| Pixel size (Å) | 0.84 | |
| Symmetry imposed | C1 | |
| Initial particle images (no.) | 889272 | |
| Final particle images (no.) | 285688 | |
| Map resolution (Å) | 2.98 | |
| FSC threshold | 0.143 | |
| Map resolution range (Å) | 2.85–4.2 | |
| Refinement | Monomer | Dimer |
| Initial model used (PDB code) | 6CFW, 4HEA | 6CFW, 4HEA |
| Model resolution (Å) | 2.97 | 3.04 |
| FSC threshold | 0.5 | 0.5 |
| Map sharpening B factor (Å) | −42, local resolution-filtered | −42 |
| Model composition | ||
| Non-hydrogen atoms | 15448 | 30250 |
| Protein residues | 1873 | 3730 |
| Ligands | 510 | 1020 |
| Waters | 242 | 0 |
| B factors (Å2) | ||
| Protein | 88.4 | 74.4 |
| Ligand | 139.1 | 123.4 |
| Waters | 89.8 | N/A |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.0080 | 0.0078 |
| Bond angles (°) | 1.32 | 1.33 |
| Validation | ||
| MolProbity score | 1.47 | 1.31 |
| Clashscore | 3.58 | 2.17 |
| Poor rotamers (%) | 0.13 | 0.10 |
| Ramachandran plot | ||
| Favoured (%) | 95.44 | 95.45 |
| Allowed (%) | 4.56 | 4.55 |
| Disallowed (%) | 0 | 0 |
| EMRinger score | 3.73 | 3.61 |