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. 2020 Jul 23;21:814–823. doi: 10.1016/j.omtn.2020.07.023

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

miR-194-5p Suppresses Osteogenic Differentiation of MSCs

(A) Quantitative real-time PCR showed that miR-194-5p expression was increased with agomiR-194-5p treatment. (B) Western blot showed that the osteogenic-related markers Runx2 and OCN were decreased with agomiR-194-5p treatment. (C and D) Quantitative real-time PCR showed that the osteogenic-related markers Runx2 (C) and OCN (D) were decreased with agomiR-194-5p treatment. (E) ALP staining of MSCs after treatment with the indicated agents for 3 days. (F) Quantification of ALP staining among the three groups. (G) Alizarin red staining of MSCs 21 days after the indicated treatment. (H) Quantification of alizarin red staining of MSCs on day 21. All data are expressed as mean ± SD. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.