Figure 1.

circRNA Profiling in Human PTX-Resistant OC Tissues and circTNPO3 Characterization

(A) Among the 723 differentially expressed circRNAs, 118 circRNAs were verified as novel circRNAs; 605 circRNAs were identified beforehand and listed in the circRNA database. (B) The 723 identified circRNAs were divided into five different categories on the basis of the way they were produced. (C) The heatmap showed the top 10 dysregulated circRNAs between PTX-resistant OC tissues and PTX-sensitive OC tissues. (D) Expression levels of top 10 dysregulated circRNAs were measured by qRT-PCR. (E) Inhibition of hsa_circ_0001741 reversed PTX resistance in both SKOV3/PTX and HeyA-8/PTX cell lines. (F) The level of circTNPO3 was significantly increased in PTX-resistant OC tissues compared to PTX-sensitive OC tissues. (G) Evaluation the diagnostic performance of circTNPO3 for OC diagnosis. (H) Kaplan-Meier curve revealed that high expression of circTNPO3 was relative to a poor overall survival in OC patients. (I) circTNPO3 expression was distinctively higher in PTX-resistant OC cells SKOV3/PTX and HeyA-8/PTX cells. All tests were performed at least three times. Data were expressed as mean ± SD. ∗∗∗p < 0.001; ∗∗p < 0.01.