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. 2020 Jun 8;21:780–791. doi: 10.1016/j.omtn.2020.06.002

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Confirmation of Subcellular Localization of circTNPO3

(A) qRT-PCR for the abundance of circTNPO3 and TNPO3 in SKOV3/PTX cells treated with Actinomycin D at the indicated time point. (B) qRT-PCR for the abundance of circTNPO3 and TNPO3 in HeyA-8/PTX cells treated with Actinomycin D at the indicated time point. (C) qRT-PCR for the expression of circTNPO3 and TNPO3 mRNA in SKOV3/PTX cells treated with or without RNase R. (D) qRT-PCR for the expression of circTNPO3 and TNPO3 mRNA in HeyA-8/PTX cells treated with or without RNase R. (E) Levels of circTNPO3 in the nuclear and cytoplasmic fractions of SKOV3/PTX cells. (F) Levels of circTNPO3 in the nuclear and cytoplasmic fractions of HeyA-8/PTX. All tests were performed at least three times. Data were expressed as mean ± SD. ∗∗p < 0.01.