Figure 7.

circTNPO3 Positively Regulated NEK2 by Interacting with miR-1299 in PTX-Resistant Ovarian Cancer Cells

(A) Venn diagram showing five genes that are putative miR-1299 targets computationally predicted by four algorithms (miRanda, RNAhybrid, miRWalk, and TargetScan). (B) mRNA levels of five candidate target genes were detected after silencing circTNPO3. (C) miR-1299 could significantly enrich the 3′ UTR of NEK2 mRNA. (D) The binding sequence between miR-1299 and NEK2. (E) Luciferase reporter assay demonstrated miR-1299 mimics significantly decreased the luciferase activity of NEK2-WT in OC cells. (F) The real-time PCR analysis demonstrated that the NEK2 was higher in PTX-resistant OC tissues. (G) The expression of NEK2 was obviously increased in PTX-resistant OC cell lines than that in their parental OC cell lines. (H) Inhibition of circTNPO3 mediated decrease of NEK2 mRNA expression was significantly recuperated following miR-1299 inhibitors. (I) Inhibition of circTNPO3-mediated decrease of NEK2 protein expression was significantly recuperated following miR-1299 inhibitors. All tests were performed at least three times. Data were expressed as mean ± SD. ∗∗p < 0.01.