Figure 7.

LNC473-miR574/miR15b-APAF1 Signaling Axis in Vivo

(A) Schematic diagram of xenografts in BALB/c nude mice by inoculating HCT116 cells. (B and C) Weights (B) and volumes (C) of tumors in xenografts from different groups. (D) Representative images of excised tumors are shown. Scale bar, 10 mm. (E) Kaplan-Meier curves showing the overall survival of nude mice from different groups. (F and G) RT-PCR (F) and qPCR (G) assays detecting miR574-5p and miR15b-5p levels upon improving LNC473 or APAF1 IRES expression in vivo. All tests were performed at least three times. Data are shown as mean ± SD. n = 6 for each group. ns, p > 0.05; ∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.