Fig. 3. Activity of the GH16 O-glycanases against porcine small intestinal mucin.

SI mucin was incubated with the GH16 enzymes alongside a broad-acting sialidase (BT0455^GH33). The control is sialidase-only. Products of small intestinal mucin digestion were labelled with procainamide at the reducing end and analysed by LC-FLD-ESI-MS. The results show a variety of oligosaccharides are released by the GH16 enzymes and majority are similar between the samples. The oligosaccharides have a variety of fucose and sulphate decorations. Species capped with α-GalNAc were determined using exo-acting enzymes specific to that sugar and linkage (see Supplementary Fig. 8).