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. 2020 Aug 11;11:4012. doi: 10.1038/s41467-020-17802-4

Fig. 4. TMBIM6 regulates AKT-dependent metabolism.

Fig. 4

a mRNA levels of glycolysis- and PPP-related genes in TMBIM6 KO and WT HT1080 cells, as determined by qRT-PCR. Quantification data represent the expression level of genes in the KO cells compared with those in normalized WT cells (n = 3 independent experiments). Data are presented as means ± SD. ****p < 0.0001, two-way ANOVA followed by Bonferroni’s post hoc test. b, c Glucose consumption and lactate production in TMBIM6 KO and WT HT1080 cells (n = 3 independent experiments). Data are presented as means ± SD. **p < 0.01; ***p < 0.001, one-way ANOVA followed by Tukey’s post hoc test. d Metabolite analysis in TMBIM6 KO and WT HT1080 cells (n = 2 independent experiments). e, f mRNA levels of GSH biosynthesis genes and de novo lipid biosynthesis genes in TMBIM6 KO and WT HT1080 cells, as determined by qRT-PCR. Quantification data represent the expression level of genes in the KO cells compared with those in normalized WT cells (n = 3 independent experiments). Data are presented as means ± SD. *p < 0.05, **p < 0.01, ****p < 0.0001, two-way ANOVA followed by Bonferroni’s post hoc test. g Immunofluorescence images of protein synthesis in TMBIM6 KO and WT HT1080 cells. Right, quantification data represent the expression intensity compared with that in normalized WT cells (n = 3 independent experiments). Data are presented as means ± SD. *p < 0.05, two-tailed unpaired t-test. Scale bar, 15 μm. h Glycosylation-related gene lists in TMBIM6 KO and WT HT1080 cells by microarray. i Glycosylated protein levels in TMBIM6 KO and WT HT1080 cells (n = 3 independent experiments). Data are presented as means ± SD. *p < 0.05, two-tailed unpaired t-test.