Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 11;7:263. doi: 10.1038/s41597-020-00594-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files associated with this article.

PMC Copyright notice

Fig. 5 — The application of CHO global spectral library in proteome profiling and quantification of HCCF and DSP mAb samples. (a) The violin plot showed the distribution of CV values across the large-scale analysis of HCCF samples. The CV values were calculated using the technical replicates for each of the six biological replicates in E01 – E06. (b) The Pearson correlation matrix of the abundance of proteins identified in the HCCF samples from experimental group E01 – E06. The median correlation coefficient r value (0.88) was represented as a red colored dash line in the color bar. (c) Heat map analysis of confidently identified proteins (at 20% CV cutoff and 1% peptide FDR in OM) in the typical DSP purification workflow. (d) The bar chart showed the average protein abundances of four well-known difficult-to-remove HCPs, including clusterin (Clu), cathepsin D (Ctsd), heat shock cognate 71 kDa protein (Hspa8) and phospholipase B-like 2 (Plbl2), identified in the DSP purification process. The error bars indicated the standard deviations between replicates. OM: original material; PrA: protein A eluate; CEX: cation exchange; AEX: anion exchange.