Table 2.

Mean Dice coefficient between randomly selected nuclei of the manual annotations and the ground truth annotations with respect to the human annotator.

Annotator	GNB-I	GNB-II	NB-I	NB-II	NB-III	NB-IV	NC-I	NC-II	NC-III	TS	Overall
Biol. exp.	0.853	0.781	0.883	0.932	0.802	0.946	0.969	0.869	0.969	0.895	0.890
Annot. exp.	0.877	0.849	0.896	0.892	0.888	0.957	0.969	0.928	0.973	0.895	0.912

Annot. exp.: annotation expert, Biol. exp.: expert biologist, GNB-I: ganglioneuroblastoma tissue sections, GNB-II: ganglioneuroblastoma tissue sections with a low signal-to-noise ratio, NB-I: neuroblastoma bone marrow cytospin preparations, NB-II: neuroblastoma cell line preparations with different magnifications, NB-III: neuroblastoma cell line preparations with different magnifications and imaged with LSM modalities, NB-IV: neuroblastoma tumor touch imprints, NC-I: normal cells (HaCaT) cytospin preparations, NC-II: normal cells (HaCaT) with low signal-to-noise ratio, NC-III: normal cells (HaCaT) grown on slide, TS: other tissue sections (neuroblastoma, Wilms tumor). Bold values set the baseline for machine learning-based image segmentation methods.