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. 2020 Aug 11;10:13553. doi: 10.1038/s41598-020-70454-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Empagliflozin modulates mRNA and protein levels of cGCH1. (a) Cardiac mRNA expression of cGCH1. (b) Representative western blot analysis of cGCH1 in border zone tissue and quantitative analysis. (c,d) Representative immunohistochemical cGCH1 staining in border zone; Scale bar: 50 μm. (e) Cardiac GCH1 activity. (f) Tetrahydrobiopterin levels. *p < 0.05, ***p < 0.001.