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. 2020 Aug 5;8:946. doi: 10.3389/fbioe.2020.00946

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Copyright © 2020 Xu, Tian, Tong, Zhang, Luo, Shang and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell apoptosis in BMSC sheet cultures. Cells from Days 1, 4, and 7 cultures were stained with Annexin V and PI, and the populations corresponding to viable and non-apoptotic (Annexin V– PI–), early (Annexin V+PI–), and late (Annexin V+PI+) apoptotic cells. (A) Representative flow cytometry graphs of cell apoptotic analysis. (B) The percentage of early apoptotic cells and the percentage of late apoptotic cells in Days 1, 4, and 7 cultures. Results are mean ± SD. All experiments were performed in triplicate. *P < 0.05 vs. Day 1 cells and ^#P < 0.05 vs. Day 4 cells.