Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 5;8:946. doi: 10.3389/fbioe.2020.00946

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Xu, Tian, Tong, Zhang, Luo, Shang and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Effect of cell sheet culture on senescence and apoptosis related gene expression. (A) Expression of cell aging related genes was tested by Real-time PCR using total RNA isolated from BMSC sheets, including p16, p21, p53, p27 and Caspase-3. Anti-aging gene expression of Bcl-2, Bax, Cyclin D1, and β-catenin. *P < 0.05 vs. Day 1 cells and ^#P < 0.05 vs. Day 4 cells. All experiments were performed in triplicate. (B) SA-β-gal staining showed that 3-day Wnt3a treatment reduced cell senescence in cell sheet cultures. (C) Western blot showed an increase of p27 and β-catenin in BMSCs after treatment with Wnt3a for 3 days. (D) Quantification of western blot bands shown in (C).