FIGURE 5.

Cocaine CPP alters the expression of circRNAs from behavior-associated genes. RNA extracted from the NAc of mice after CPP testing was subjected to circRNAs arrays as described in section “Materials and Methods.” (A) Differentially expressed circRNAs were analyzed to identify Top Diseases and Functions (FC > 1.2, p < 0.05). n = 3 for each condition (Saline control, Cocaine). (B) Representation of molecules and functions from behavior-associated circRNAs. Molecules in blue are downregulated and in red are upregulated. Blue arrows indicate the molecule is inhibiting the pathway and gray arrows indicate the lack of information on the pathway interaction. (C) Molecular functions associated with top circRNA changes (FC > 1.3, p < 0.05). (D) Representation of molecules and functions from the Cellular Growth and Proliferation-associated circRNAs in panel (C). Molecules in blue are downregulated, in red are upregulated; color intensity is proportional to the fold change in expression levels after CPP. Blue arrows indicate the molecule is inhibiting the pathway, while red arrows indicate activation.