Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 27;12:6373–6383. doi: 10.2147/CMAR.S256490

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Bang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

KLF16 silencing inhibited breast cancer cell proliferation in vitro and in vivo. MCF-7 and MDA-MB-231 cells were transfected with two different KLF16-targeting shRNAs or control shRNA (shSCR). (A) The knockdown efficiency was confirmed with Western blotting. β-Actin was chosen as an internal control in Western blotting. (B) CCK-8 assay was used to assess the viability of each cell group at the indicated time points. (C) Colony formation assay in MCF-7 and MDA-MB-231 cells expressing the indicated shRNAs. (D) The numbers of colonies in each group were counted. Each value represents the mean ± standard deviation of three independent experiments in (B and D); ***p<0.001. (E) The images of xenografts are shown. (F) The final tumor volumes are shown. (G) The tumor weights are shown. Bars in F and G represent mean ± standard deviation (Mann–Whitney, **p <0.01, n=5).