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. 2020 Jul 27;12:6373–6383. doi: 10.2147/CMAR.S256490

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© 2020 Bang et al.

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KLF16 silencing impairs migration and invasion and suppresses EMT in breast cancer cells. (A) MCF7 and MDA-MB-231 cells expressing KLF16-targeting shRNAs or control shRNA were used for transwell migration and invasion assays. Representative images after 0.1% crystal violet staining are shown. (B) The quantitative results of migration and invasion for each group are shown; Bar graphs represent mean ± standard deviation of three independent experiments (t-test, *** p<0.001). (C) The protein levels of ZEB1, E-cadherin, MMP9, Slug, and KLF16 were assessed by Western blotting. β-Actin was used as the internal control in Western blotting analysis.