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. 2020 Aug 5;11:949. doi: 10.3389/fphar.2020.00949

Figure 2.

Figure 2

Effect of cinnamic aldehyde (CA) on lipopolysaccharide (LPS)-induced interleukin (IL)-1β, IL-6, and tumor necrosis factor-alfa (TNF-α) expression in human osteoarthritis (OA) chondrocytes. Human OA chondrocytes were pretreated for 24 h with various concentrations of CA (10, 20, and 50 μM) and then stimulated or not stimulated with LPS (10 μg/ml) for 24 h. (A) Messenger RNA (mRNA) expression levels of IL-1β, IL-6, and TNF-α were determined using real-time quantitative polymerase chain reaction (RT-qPCR). (B) Enzyme-linked immunosorbent assay results for IL-1β, IL-6, and TNF-α in chondrocyte supernatants. (C, D) Protein expression levels were assayed by Western blot and quantification analysis using Image J software. #P < 0.01 compared with control group; *P < 0.05, **P < 0.01 compared with LPS group. All experiments were performed at least 3 times independently ( n = 3).