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Palmitic acid treatment had low cytotoxicity in GS cells. (A) WST-1 assay suggesting the effect of intracellular fat accumulation (dose-dependent) on cellular cytotoxicity of GS cells in culture. Cell viability is expressed as % of control cells (1% BSA). (B) Fluorescence microscopy showing the accumulation of lipids intracellular at 24 h post-treatment by Nile Red staining (red). Cell nuclear was stained (blue) using Hoechst33342. The images were taken at 40× magnifications.
