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. 2020 Jul 9;15(2):317–325. doi: 10.1016/j.stemcr.2020.06.011

Figure 2.

Figure 2

Human Aging Is Associated with Increased Adjacency between CD34+ HSPCs and Adipocytes

(A) Positive correlation between adipocytes and CD34+ HSPCs. Pearson correlation test, r = 0.56, n = 10.

(B) Representative images of CD34+ HSPCs spatially associated with adipocytes in BM biopsies from hematologically healthy patients aged 50–64 and 65–92 years. From left to right, CD34+ HSPCs IHC image, CD34+ objects’ detection image and digitalized image showing cells not adjacent (in blue) and immediately adjacent to adipocytes (in green and red). Scale bar, 200 μm.

(C) Bar graph showing number of CD34+ HSPCs not adjacent and immediately adjacent to adipocytes: 50–64 years (n = 6) versus 65–92 years (n = 4), two-way ANOVA, p = 0.01, unpaired t test, immediately adjacent to adipocytes, p = 0.01.

(D) Bar graph showing number of adipocytes immediately adjacent to 1 or >1 CD34+ HSPCs: 50–64 years (n = 6) versus 65–92 years (n = 4), two-way ANOVA p = 0.052, unpaired t test, >1 in both age groups, p = 0.05.

(E) Bar graph showing CD34+ HSPC distance to vessels. Graph representing percentage of CD34+ HSPCs at a distance of 0–10, 10–20, and >20 μm from vessels: 50–64 years (n = 3) versus 65–92 years (n = 3), two-way ANOVA p = 0.27.

(F) Representative IMC images showing an overlay of CD34+ HSPCs (red channel), NGFR+ BMSCs (green channel), and adipocytes (blue channel) in BM biopsies from hematologically healthy patients aged 50–64 (n = 2) and 65–92 years (n = 3). Scale bar, 100 μm. Adjacency between CD34+ HSPCs, NGFR+ BMSCs, and adipocytes are indicated by white arrows.