Cell-Type-Specific and Heterogeneous GATA2 Protein Expression in Adult HSPCs
Flow-cytometric quantification of GATA2VENUS expression in adult HSPCs. GATA2VENUS gate was set using wild-type cells (see Figure S5). Numbers in quadrants represent percentage of cells. See Figure S3 for gating schemes for these cell types: HSC, hematopoietic stem cell; MPP 1–4, multipotent progenitors 1–4; P.MegE, pre-megakaryocyte-erythrocyte; MkP, megakaryocyte progenitor; CFUE, colony-forming unit erythrocyte; CD71highTER119low and CD71lowTER119high, erythrocyte differentiation stages; preGM, pre-granulocyte-monocyte; GMP, granulocyte-monocyte progenitor; MDP, monocyte dendritic cell progenitor; MP, monocyte progenitor; cMoP, common monocyte progenitor; GP, granulocyte progenitor; EoP, eosinophil progenitor; preBMP, pre-basophil mast cell progenitor; MCP, mast cell progenitor; BaP, basophil progenitor.