Figure 4.

GATA2 Protein Expression Identifies preGM and GMP Subpopulations with Differential Hematopoietic TF Expression

(A) GATA2-negative and -high preGMs and GMPs were sorted before immunostaining, imaging, and quantification of core hematopoietic TFs.

(B) Representative fluorescence images of preGMs (left panel) and GMPs (right panel) stained with DAPI and anti-TF antibodies. Scale bar: 50 µm.

(C) Quantification of TF levels in GATA2-negative and -high preGMs and GMPs. Data represented by box and whisker plots with median (n = 3 independent mice). Two-tailed t test; ^∗∗∗p < 0.001, ^∗∗p < 0.01, ^∗p < 0.05.