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. 2020 Jul 9;15(2):326–339. doi: 10.1016/j.stemcr.2020.06.008

Figure 5.

Figure 5

GATA2 Protein Levels Identify Early Segregation of Monocyte and Mast Cell Lineages but Not Neutrophils

(A) GATA2-negative and -high preGMs and GMPs were sorted and cultured in pan-myeloid medium (IMDM + FCS + BIT + SCF + GM-CSF + IL-3 + IL-9).

(B) Quantification of mature cell types at day 8 by flow cytometry. GATA2-negative preGMs and GMPs mainly generate CD11b+ CD115+ FCεR1− monocytes and CD11b+ LY6G+ FCεR1− neutrophils while GATA2-high preGMs and GMPs generate cKIT+ FCεR1+ CD11b− CD115− LY6G− mast cells and few neutrophils. Numbers in quadrants represent percentage of cells.

(C) Representative images of May-Grünwald Giemsa morphology of cells from day 8 culture preGMs. GATA2-negative versus -high preGMs generate monocytes and neutrophils versus mast cells and neutrophils, respectively. Scale bar, 20 μm.