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. 2020 Aug 5;10:1371. doi: 10.3389/fonc.2020.01371

Figure 4.

Figure 4

Effect of miR-27b-3p on cell proliferation and invasion depends on its inhibitory effect on PPARG expression in TNBC cells. The MDA-MB-231 cells were transfected with miR-27b-3p-3p NC, miR-27b-3p-3p mimics, or miR-27b-3p-3p mimics and treated with PPARG agonist (rosiglitazone, 80 μmol/L) for 24 h. (A) CCK-8 test assay showed that overexpression of miR-27b-3p could promote cell proliferation. However, this promotion effect of miR-27b-3p could be reversed by PPARG agonist. (B,C) Wound healing assay showed that PPARG agonist abolished the effect of miR-27b-3p mimics on the wound-healing rate of MDA-MB-231 cells. (D,E) Transwell invasion assay. The number of invasive cells in the miR-27b-3p mimics group were significantly higher than those of the control group, which were significantly decreased by PPARG agonist. For (A,C,E), results are shown as mean ± SD of 3 times of experiments in each group. ***P < 0.001; NC, negative control of transfection vectors.