Figure 1.

Tissue- and cell-specific localization of ANN1-GFP in different aerial organs of A. thaliana seedlings stably expressing proANN1::ANN1:GFP construct using confocal laser scanning microscopy (CLSM). (A) Expression of ANN1-GFP in hypocotyl epidermal cells showing its localization in the cell cortex, cytoplasmic strands, and in areas around membranous compartments resembling ER bodies (arrows). ANN1-GFP expression delineated cell cortex in stomata guard cells (s) and subsidiary cells (sb). (B) Unequal distribution of ANN1-GFP in epidermal cells of developing first true leaf. Prominent production of ANN1-GFP was evident in expanding pavement cells (p), whereas it was almost absent in stomatal precursor cells: meristemoid mother cells (red asterisks), meristemoids (white arrowhead) and guard mother cells (white asterisks). (C, D) Distribution of ANN1-GFP within radially organized arrays of cytoplasmic strands in leaf stomata guard cells presented in the medial plane (C) and in the cortical plane (D). Note the absence of ANN1-GFP fluorescence in nuclei indicated by yellow arrowheads in (B, C). (E, F) Color-coded images of ANN1-GFP localization in different developmental stages of trichomes. Note the tip-focused accumulation of ANN1-GFP in trichomes (E, F) and its lower abundance in basal cells of developed trichome indicated by an arrow in (F). Scale bars = 20 μm.