Figure 5.

In vivo localization of ANN1-GFP during lateral root formation and emergence in transgenic A. thaliana expressing proANN1::ANN1:GFP construct using LSFM. (A) Representative images of selected developmental stages of lateral root primordium (LRP) establishment. ANN1-GFP signal was localized in the cytoplasm and was absent in nuclei of LRP cells in early stages of LRP formation (phases I, III, and V). ANN1-GFP signal considerably decreased in LRP cells in the phase VII and during lateral root emergence. (B) Cross-section of the primary root from orthogonal projection before LRP initiation with the strong and uniformly distributed signal of ANN1-GFP in the stele (st) and decreased amount in vacuolated cells of endodermis (en), cortex (cor), and epidermis (ep). (C) Drop of the ANN1-GFP signal intensity in the pericycle and adjacent stele tissue at the time of LRP establishment. (D) Distribution of ANN1-GFP signal in the emerging lateral root. Tissue-specific fluorescence intensity in emerging lateral root was determined along the profile representing a cross-section of the primary root and longitudinal gradient in developing lateral root (as shown by dotted red arrow). (E) The fluorescence intensity along the profile shown in (D). Scale bars = 50 μm.