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. 2020 Jul 16;15(2):424–438. doi: 10.1016/j.stemcr.2020.06.013

Figure 1.

Figure 1

Expression Profiles of JMJD1A and JMJD1B in Prospermatogonia

(A–C) P3 wild-type mouse testes sections stained with an anti-H3K9me2 antibody. Sertoli cells and Leydig cells were marked with antibodies against SOX9 (A) and HSD3β (B), respectively. A TRA98 antibody was used to detect germ cells (C). Nuclei were stained with DAPI. Dashed lines represent basement membranes. Asterisks represent germ cells. Scale bars, 20 μm.

(D) Quantitative mRNA analysis for Jmjd1a (left) and Jmjd1b (right) in germ cells and somatic cells separated from Oct4-EGFP Tg testes at the indicated time points. Relative mRNA levels in E13.5 somatic cells were arbitrarily defined as 1. Data are presented as the mean ± SD (n = 3 mice for each time point).

(E and F) Immunofluorescence analysis of JMJD1A and JMJD1B in embryonic testes sections. Dashed lines represent basement membranes. Asterisks represent germ cells. Scale bars, 50 μm. (E) E17.5 wild-type testis sections were stained with an anti-JMJD1A antibody and DAPI. Enlarged boxes represent double staining for JMJD1A and DAPI (top) and single staining for JMJD1A (bottom). (F) E17.5 Jmjd1b+/FLAG−KI testes were stained with an anti-FLAG antibody and DAPI. Enlarged boxes represent double staining for FLAG and DAPI (top) and single staining for FLAG (bottom).