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. 2020 Jul 16;15(2):424–438. doi: 10.1016/j.stemcr.2020.06.013

Figure 2.

Figure 2

JMJD1A and JMJD1B Catalyze H3K9me2 Demethylation in Prospermatogonia

(A and B) Immunoblot analysis of JMJD1A (A) and JMJD1B (B) in neonatal testicular cells. Germ cells and somatic sells were separated from P0 testes of the indicated genotypes for immunoblot analysis. Enrichment of germ cells was confirmed by immunoblotting with an anti-VASA antibody. Ponceau staining was used as a loading control.

(C) Immunofluorescence analysis of H3K9me2 in P3 testes sections from the indicated genotypes. A TRA98 antibody was used to detect germ cells. Scale bars, 5 μm.

(D) Relative fluorescence intensities of H3K9me2 in P3 germ cells of the indicated genotypes. Fluorescence intensities of germ cells and neighboring Sertoli cells were measured using ImageJ software. Relative fluorescence intensity values were calculated by dividing the fluorescence intensities of germ cells by those of Sertoli cells. We examined >50 germ cells per genotype. ∗∗∗p < 0.001 (one-way ANOVA with Tukey's test).