Figure 1.

UTX Is Upregulated in Earlier Stages of Neural Differentiation of hESCs

(A) Schematic illustration of neural differentiation of hESCs. hESCs, human embryonic stem cells; EB, embryoid bodies; hNSCs, human neural stem cells; hNPCs, human neural progenitor cells. Scale bar, 50 μm.

(B) Real-time PCR analysis showing higher mRNA levels of UTX in earlier stages of neural differentiation of hESCs. GAPDH is used as an internal control for real-time PCR.

(C) Western blotting indicating upregulated protein levels of UTX at earlier stages during neural differentiation of hESCs. β-Tubulin is used as a loading control.

(D) Representative images of H3K27me3 immunostaining at different stages of neural differentiation of hESCs. DAPI, a nuclei marker; Nestin, an NSC marker; DCX, an NPCs marker; MAP2, a mature neuron marker. Scale bar, 50 μm.

(E) Western blotting showing protein levels of H3K27me3 and H3K27ac upon neural differentiation of hESCs. Histone 3 was used as a loading control.

Results are presented as mean ± SEM; n ≥ 3 independent experiments. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.