Figure 6.

Persistent AMPK Activation in iPSC-Derived Cardiomyocytes Changes Gene Expression through Chromatin Accessibility

(A) Heatmap of motif scores on cardiac- or metabolic-related transcription factors.

(B) Volcano plots showing differential motif scores of cardiac and metabolic transcription factors.

(C) Comparison of chromatin accessibility between NAM only (top two replicates) and AICAR only (bottom two replicates) to control at TNNT2, TNNI1, TNNI3, MYH6, and MYH7 loci. The peak heights are normalized to the ACTB locus.

(D) MFI of DAPI in control and AICAR-treated Gibco-CMs, n = 5 wells with 10 fields/well; ^∗∗∗∗p < 0.0001 unpaired t test.

(E) MFI by flow cytometry for DAPI in control and AICAR, n = 3 I.E.; ^∗∗p < 0.001 unpaired t test.

(F) Unsupervised hierarchical clustering of cardiac gene expression from NanoString analysis, n = 3 independent biological replicates.

(G) Volcano plots showing differential gene expression analysis of cardiac genes.