Figure 1.

The Number and the Length of Primary Cilia Are Reduced in the DG of Fmr1 KO Mice

(A) Schematic view of the hippocampus (CA1, CA3, and DG), and the cortex (S1 and EC), where primary cilia were investigated.

(B and C) Left: immunostaining of (B) AC3 (magenta) or (C) Arl13b (magenta) with DAPI nuclear staining (green) in the DG of P60 WT or Fmr1 KO mice. Higher magnifications of the left images (yellow boxes) are shown in the middle and right panels. Right: quantification of the percentage of (B) AC3⁺ or (C) Arl13b⁺ cells among DAPI⁺ cells in the DG.

(D) Quantification of the length of AC3⁺ primary cilia in the DG of P60 WT or Fmr1 KO mice.

n = 8, mean ± SEM, Student's unpaired t test. ^∗∗p < 0.01, ^∗∗∗∗p < 0.0001. See also Figure S1.