Figure 3.

Primary Cilia Loss in the DG of Fmr1 KO Mice Is Age Dependent

Brain sections were prepared from WT or Fmr1 KO mice at E18.5, P0, P7, P10, P14, P30, and P60.

(A) Immunostaining of AC3 (magenta) with DAPI nuclear staining (green) in the DG of WT or Fmr1 KO mice. AC3⁺ primary cilia are shown in upper panels of WT and Fmr1 KO mice, and AC3⁺ primary cilia with DAPI⁺ cells are shown in lower panels of WT and Fmr1 KO mice.

(B) Quantification of the percentage of AC3⁺ cells among DAPI⁺ cells in the DG of WT and Fmr1 KO mice at E18.5, P0, P7, P10, P14, P30, and P60.

n = 8, mean ± SEM, Student's unpaired t test. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗∗p < 0.0001.