Figure 4.

Age-Dependent Primary Cilia Deficits Are Found Specifically in NeuN⁺ Mature Granule Neurons of Fmr1 KO Mice

(A) Immunostaining of AC3 (magenta) and NeuN (cyan) in the DG of P7, P10, P14, P30, and P60 WT or Fmr1 KO mice. AC3⁺ primary cilia are shown in upper panels of WT and Fmr1 KO mice, and AC3⁺ primary cilia with NeuN⁺ cells are shown in lower panels of WT and Fmr1 KO mice. White arrows, AC3⁺;NeuN⁺ cells; yellow arrows, AC3⁺;NeuN⁻ cells.

(B and C) Quantification of the percentage of AC3⁺ cells among (B) NeuN⁺ cells or (C) NeuN⁻ cells. n = 8, mean ± SEM.

Student's unpaired t test. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗∗p < 0.0001. See also Figures S2 and S3.