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. 2020 Jul 30;15(2):454–466. doi: 10.1016/j.stemcr.2020.07.001

Figure 5.

Figure 5

No Significant Change in the Number of Primary Cilia Is Found in NPCs (BLBP+) or Astrocytes (S100β+) in the DG of Fmr1 KO Mice

(A) Immunostaining of Arl13b (magenta) and BLBP (cyan) with DAPI nuclear staining (gray) in the DG of P30 and P60 WT or Fmr1 KO mice. White arrows, Arl13b+;BLBP+ cells.

(B) Quantification of the percentage of Arl13b+ cells among BLBP+ cells.

(C) Gli1 mRNA level was analyzed by qRT-PCR in the DG of adult WT or Fmr1 KO mice. Values of Gli1 mRNA were normalized by housekeeping gene peptidylprolyl isomerase A (Ppia) mRNA levels.

(D) Immunostaining of AC3 (magenta) and S100β (green) with DAPI nuclear staining (gray) in the DG of P7, P14, P30, and P60 WT or Fmr1 KO mice. White arrows, AC3+;S100β+ cells.

(E) Quantification of the percentage of AC3+ cells among S100β+ cells. n = 8 for immunostaining, n = 3 for qRT-PCR, mean ± SEM. Student's unpaired t test.