Figure 6.

Newborn Neurons (NeuN⁺;BrdU⁺) Originated from the SGZ Exhibit Primary Cilia Loss in the DG of Fmr1 KO Mice

BrdU was administrated by intraperitoneal injection into WT or Fmr1 KO mice at E12.5, E15.5, P7–8, P30–31, or P60–61 followed by brain collection at 28 days post injection (dpi).

(A) Immunostaining of AC3 (magenta), NeuN (gray), and BrdU (green) in the DG of BrdU-treated WT or Fmr1 KO mice. Orange arrows, AC3⁺;NeuN⁺;BrdU⁺ cells. Inner layer (toward hilus) of the DG is located on the lower side of images.

(B) Schematic view of BrdU treatment and collection of WT or Fmr1 KO mice.

(C) Quantification of the percentage of AC3⁺ cells among NeuN⁺;BrdU⁺ newborn neurons originating at E12.5, E15.5, P7–8, P30–31, or P60–61 in WT and Fmr1 KO mice (28 dpi: P21, P24, P36, P59, or P89).

n = 8–10, mean ± SEM, Student's unpaired t test. ^∗p < 0.05, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001. See also Figures S4–S6.