Figure 6.

Better DNA Damage Repair in HSCs from Postn^−/− FL Tissues

(A–D) Postn^+/+ and Postn^−/− FL-derived LSK cells were used for assessing the expression of DNA damage response genes through qRT-PCR. Expression of genes that belong to MMR, such as Exo1, Pcna, Setd2, and Rpa3 (A), NER, such as Cetn2, Ddb1, and Ercc1 (B), BER, such as Tdg, Neil1, Neil3, and Xrcc1 (C), and HR pathways, such as Topbp1, Palb2, Brca1, and Rad51 (D) was examined. Gene transcript changes were normalized to β-actin levels and relative expression of respective genes was examined in LSK cells from Postn^−/− FL tissues as compared with the Postn^+/+ FL-derived cells. Unpaired two-tailed Student's t test. n = 4–6. ^∗p < 0.0055.

(E and F) Freshly sorted primitive HSCs (CD150⁺CD48⁻ LSK cells) from E14.5 Postn^+/+ and Postn^−/− FL tissues were used to perform neutral comet assay. (E) Representative comets from the HSCs from Postn^+/+ (upper panel) and Postn^−/− (lower panel) FL tissues. Scale bar, 10 μm. (F) Olive tail moment compared between Postn^+/+ (N = 379) and Postn^−/− (N = 307) FL HSCs. n = 4–6, Mann-Whitney U test: ^∗p = 0.0407.