Figure 1.

Mthfd2 Is Important for mESCs to Maintain Self-Renewal

(A) Representative results of Mthfd2 KD mESCs with AP staining. Scale bars, 100 μm.

(B and C) qRT-PCR analysis of mRNA levels of pluripotency marker genes (B) and lineage marker genes (C) in Mthfd2 KD mESCs.

(D) Representative results of Mthfd2 KO mESCs with AP staining. Scale bars, 200 μm.

(E and F) qRT-PCR analysis of mRNA levels of pluripotency marker genes (E) and lineage marker genes (F) in Mthfd2 KO mESCs.

(G) Representative growth curve of Mthfd2 KO mESCs.

(H) Representative results of overexpressed (OE) Mthfd2-Mthfd2 KO mESCs with AP staining. Scale bar, 200 μm.

(I and J) qRT-PCR analysis of mRNA levels of Mthfd2 (I) and pluripotency marker genes (J) in OE Mthfd2-Mthfd2 KO mESCs.

(K) Representative growth curve of OE Mthfd2-Mthfd2 KO mESCs.

(L) Western blot analysis of the levels of the MTHFD2 protein during differentiation of mESCs. GAPDH was used as a loading control.

Data in (B), (C), (E), (F), (I) and (J) are pooled from three independent experiments (mean ± SD) relative to EF1-α and the control mESCs. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 (Student's t test) compared with the control.

See also Figure S1.