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. 2020 Jul 16;15(2):529–545. doi: 10.1016/j.stemcr.2020.06.018

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Mthfd2 Deficiency Induces Mitochondrial Dysfunction by Reducing Uqcrc2 Expression

(A) Measurements of the mitochondrial membrane potential (MMP) in Mthfd2 KD mESCs using tetramethylrhodamine methyl ester (TMRE).

(B) Total ATP levels in Mthfd2 KD mESCs.

(C) Flow-cytometry analysis of ROS levels in Mthfd2 KD mESCs.

(D) Measurements of the MMP in antimycin A-treated mESCs (AA-mESCs) using TMRE.

(E) Flow-cytometry analysis of ROS levels in AA-mESCs.

(F) Measurements of the MMP in Uqcrc2 KD mESCs using TMRE.

(G) Flow-cytometry analysis of superoxide levels in Uqcrc2 KD mESCs.

(H) Total ATP levels in Uqcrc2 KD mESCs.

(I and J) Examination of intracellular glucose levels (I) and lactate levels (J) in Uqcrc2 KD mESCs.

(K) Measurements of the MMP in OE Uqcrc2-Mthfd2 KD mESCs using TMRE.

(L) Flow-cytometry analysis of ROS levels in OE Uqcrc2-Mthfd2 KD mESCs.

All data are pooled from three independent experiments (mean ± SD). ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 (Student's t test) compared with the control. A representative histogram (left) and quantification of the mean fluorescence intensity (right) are presented for (A), (C) to (G), (K), and (L). See also Figure S5.