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. 2020 Jul 16;15(2):529–545. doi: 10.1016/j.stemcr.2020.06.018

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Mthfd2 Regulates Pluripotency of mESCs by Modulating both Mitochondrial Function and DNA Repair

(A) Representative results of Uqcrc2-Exo1 KD mESCs with AP staining. Scale bars, 100 μm.

(B) qRT-PCR analysis of mRNA levels of Uqcrc2 and Exo1 in Uqcrc2-Exo1 KD mESCs.

(C and D) qRT-PCR analysis of mRNA levels of pluripotency marker genes (C) and lineage marker genes (D) in Uqcrc2-Exo1 KD mESCs.

(E) Representative results of OE Uqcrc2-, OE Cdk1- or co-OE Uqcrc2, and Cdk1-Mthfd2 KD mESCs with AP staining. Scale bars, 200 μm.

(F) qRT-PCR analysis of mRNA levels of Uqcrc2, Cdk1, and Mthfd2 in OE Uqcrc2-, OE Cdk1- or co-OE Uqcrc2, and Cdk1-Mthfd2 KD mESCs.

(G and H) qRT-PCR analysis of mRNA levels of pluripotency marker genes (G) and lineage marker genes (H) in OE Uqcrc2-, OE Cdk1- or co-OE Uqcrc2, and Cdk1-Mthfd2 KD mESCs.

Data in (B) to (D) and (F) to (H) are pooled from three independent experiments (mean ± SD) relative to EF1-α and the control. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 (Student's t test) compared with the control. See also Figure S7.