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. 2020 Aug 3;133(15):jcs246033. doi: 10.1242/jcs.246033

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Knocksideways indicates no visible role for retromer in SNX-BAR mediated retrograde transport of CI-MPR. (A) Retromer knocksideways HeLa cells were fixed before or at multiple time points after the addition of rapalog and labelled for Golgin97 and CI-MPR. A merged panel displays both the anti-Golgin97 and anti-CI-MPR channels with a magnified image (inset). (B) Pearson's colocalisation between CI-MPR and Golgin97 at multiple timepoints after the addition of rapalog. n_exp=3, n_cell=60 with all datapoints being displayed. (C) Retromer knocksideways HeLa cells were fixed before or at multiple time points after the addition of rapalog and labelled for TGN46 and CI-MPR. A merged panel displays both the TGN46 and CI-MPR channels with a magnified image (inset). (D) Pearson's colocalisation between CI-MPR and TGN46 at multiple timepoints after the addition of rapalog. n_exp=3, n_cell=60 with all datapoints being displayed. N/S, not significant (P>0.05) (ordinary one-way ANOVA with multiple comparisons). Error bars show the s.e.m. Scale bars: 10 µm.