Fig. 3.

Increased number of microglial precursors and differentiating microglia in Npc1^nmf164 mice at P10. (A) Images of P10 whole cerebellum sections immunostained with IBA1 showing the distribution and abundance of IBA1⁺ cells in WT and Npc1^nmf164 mice. Boxes indicate some regions of WM. (B) High magnification images of the WMR immunostained with IBA1 and KI67 showing higher number of IBA1⁺ cells in the Npc1^nmf164 mouse. AT, axonal tract. (C) High magnified images in a region of the cerebellar cortex immunostained with IBA1 and KI67 showing higher number of IBA1⁺ cells in the Npc1^nmf164 mouse. (D) Quantification of the volume of IBA1⁺ microglia clusters in the cerebellar WMR. (E) No differences were found in the percentage of WM IBA1⁺/KI67⁺ cells between WT and Npc1^nmf164 mice. (F) Quantification of the number of IBA1⁺ cells in the PCL/ML and PS. (G) Quantification of the number of IBA1⁺/KI67⁺ cells in the PCL/ML and PS. Data are presented as mean±s.e.m. (D,E) n=2 images per mouse, n=4 mice; (F,G) n=4 mice. *P<0.05. n.s., not significant; u.a., unit area. Scale bars: 250 µm (A); 30 µm (B,C).