Table 3.
Relative quantitation of blaOXA-51 and blaOXA-58 mRNA level and β-lactamase activity in five A. baumannii isolates.
| Isolate | DN050 | TN078 | DN014 | TN341 | TN345 | |
|---|---|---|---|---|---|---|
| MIC imipenem (μg·ml−1) | ≥32 | 0.5 | 0.19 | 0.75 | 0.5 | |
|
| ||||||
| Relative expression of blaOXA-51 | ΔCt | 8.87 ± 0.39 | 12.87 ± 0.30 | 7.54 ± 0.66 | 9.64 ± 0.71 | 8.80 ± 0.60 |
| Expression (time) | 1.00 (0.76-1.31) |
0.06 (0.05-0.08) |
2.51 (1.58-3.98) |
0.59 (0.36-0.96) |
1.04 (0.69-1.59) |
|
|
| ||||||
| Relative expression of blaOXA-58 | ΔCt | 4.18 ± 1.18 | 8.64 ± 0.48 | 7.99 ± 2.23 | 8.45 ± 0.53 | 7.28 ± 0.24 |
| Expression (time) | 25.69 (11.3-58.37) |
1.17 (0.84-1.64) |
1.84 (0.39-8.61) |
1.33 (0.93-1.93) |
3.01 (2.55-3.56) |
|
|
| ||||||
| Total β-lactamase activity (U·mg−1) | Extracellular | 10.8 ± 3.3 | 17.7 ± 5.2 | 13.9 ± 4.1 | 10.8 ± 3.3 | 12.9 ± 3.8 |
| Periplasmic | 44.7 ± 12.8 | 15.3 ± 4.3 | 28.5 ± 8.2 | 49.6 ± 16.0 | 27.4 ± 10.0 | |