Fig. 2.

Effect of SIRPB1 knockdown on prostate cancer cell proliferation and migration in vitro. A. Immunoblot of SIRPB1 protein expression in PC3 cells transfected with control (si-Control) and two different SIRPB1 siRNAs (siSIRPB1–1, siSIRPB1–2) and mock control. B. PC3 cells transfected with control (si-Control), SIRPB1 siRNA (siSIRPB1–1, siSIRPB1–2) or mock control and reseeded in 100 mm dishes in complete media. Media were replenished every 3 days and the cells were stained with crystal violet after 10 or 14 days. The size of cells (upper) and the total number of cells (lower) were quantitated. C. Quantification of colony number. D. Quantification of colony diameter. E. Scratch assay was performed at 72 h post-transfection and images were captured at the time points indicated. F. Quantification of gap closure at 15 h and 24 h. G. Boyden chamber transwell migration assay of PC3 cells following siRNA knockdown and after 22 h with 20% FBS as chemoattractant. H. Quantification of number of migrated cells. Images are representative three independent experiments. Data are presented as mean ± SD. ***P<0.001, ****P<0.0001 compared with si-Control groups.