Fig. 4.

A. Immunoblot of SIRPB1 protein expression in C4–2 cells transfected with GFP-SIRPB1 (GFP-SIRPB1) or GFP vector (GFP). B. The above C4–2 cells were cultured in 100 mm dishes in complete media. Media were replenished every 3 days and the cells were stained with crystal violet after 10 or 14 days in colony formation assay (left panels, center panel) and Boyden chamber transwell migration assay (right panels). Migrated cells were photographed after 22 h culture with 20% FBS as chemoattractant. C. Quantification of colony number. D. Quantification of colony diameter. E. Quantification of migrated cells in transwell assay. F. Tumor incidence in mice 15 days after inoculation of C4–2 cells stably expressing GFP-SIRPB1 or GFP. Data presented are mean ± SD. ****P<0.0001.