Figure 6.

Compound JJ-450 does not induce apoptosis in C4–2 GFP-AR^WT and C4–2 GFP-AR^F876L sublines. (A) Western blot analysis of cleaved-PARP and cleaved-caspase3 in C4–2 GFP-AR^WT. Cells were treated with DMSO, 10 μM Enz, 10 μM JJ-450 or 5 nM docetaxel for 48 h prior to western blot analysis. (B) Western blot analysis of cleaved-PARP and cleaved-caspase 3 in C4–2 GFP-AR^F876L. Cells were treated the same as in (A). Results are representative of three separate experiments. Enz, enzalutamide.