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. 2020 Aug 2;2020:9125752. doi: 10.1155/2020/9125752

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Copyright © 2020 Qian Liu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of GPS on mitophagy in yeast. (a) Fluorescent images of YOM38 yeast containing plasmid pR316-GFP-Atg8 after treatment of GPS through MitoTracker Red CMXRos staining. (b) The percentage of YOM38 cells containing plasmid pR316-GFP-Atg8 with the colocation of autophagosome (green) and MitoTracker Red CMXRos (red). (c, d) The changes in ATG8-GFP, free GFP, and ubiquitin at the protein level in the mitochondria after treatment of GPS. Three pictures containing more than 60 cells in each group are used for statistical analysis.